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pdh a1146  (ABclonal Biotechnology)


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    Structured Review

    ABclonal Biotechnology pdh a1146
    Pdh A1146, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-pdh/LDHA+Rabbit+pAb/pm42623915-60-9-13
    Average 96 stars, based on 83 article reviews
    pdh a1146 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Control:

    Article Title: High-fat diet-induced obesity primes fatty acid β-oxidation impairment and consequent ovarian dysfunction during early pregnancy
    Article Snippet: Finally, an enhanced chemiluminescent (ECL) detection kit (Millipore, Germany) was employed to visualize the bands on the membranes. .. The light density and grey level were analyzed and calculated by using ImageJ (Bio-Rad, USA), with β-actin used as a loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Peptide/protein target Species Manufacturer, catalog The concentration of IHC The concentration of Western Blotting Aromatase (CYP19A1) Rabbit AB clonal, ab18995 1:200 1:1,000 Cytochrome P450 family 11 subfamily A member 1 (CYP11A1/P450Scc) Rabbit Boster, BA2120 1:100 1:500 Steroidogenic acute regulatory protein (StAR) Rabbit Bioss, bs-3570R 1:100 1:1,000 Hypoxia-inducible factor 1-alpha (HIF-1α) Rabbit AB clonal, A11945 1:100 1:1,000 Pyruvate dehydrogenase phosphatase1 (PDP1) Rabbit AB clonal, A6332 1:50 1:500 Phosphoinositide-dependent kinase1 (PDK1) Rabbit Boster, BA4499 1:100 1:1,000 Pyruvate dehydrogenase E1 alpha 1 (PDHA1) Rabbit AB clonal, A1895 1:500 1:5,000 Carnitine palmitoyltransferase 1A (CPT1A) Rabbit Proteintech, 15184-1-AP 1:1,000 β-Actin Mouse Boster, BM0005 1:1,000 Open in a separate window caption a8 Antibodies used for western blotting and immunohistochemistry (IHC) Biochemical analyses Blood was centrifuged at 3,000 rotations per minute for 10 minutes under 4 °C to obtain the serum. .. The serum levels of triglyceride (TG) and total cholesterol (TC) were determined using specific assay kits (Nanjing Jiancheng, Nanjing, China) and applying glycerol-3-phosphate oxidase p-aminophenol (GPO-PAP) and cholesterol oxidase-peroxidase coupling (COD-PAP) methods.

    Concentration Assay:

    Article Title: High-fat diet-induced obesity primes fatty acid β-oxidation impairment and consequent ovarian dysfunction during early pregnancy
    Article Snippet: Finally, an enhanced chemiluminescent (ECL) detection kit (Millipore, Germany) was employed to visualize the bands on the membranes. .. The light density and grey level were analyzed and calculated by using ImageJ (Bio-Rad, USA), with β-actin used as a loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Peptide/protein target Species Manufacturer, catalog The concentration of IHC The concentration of Western Blotting Aromatase (CYP19A1) Rabbit AB clonal, ab18995 1:200 1:1,000 Cytochrome P450 family 11 subfamily A member 1 (CYP11A1/P450Scc) Rabbit Boster, BA2120 1:100 1:500 Steroidogenic acute regulatory protein (StAR) Rabbit Bioss, bs-3570R 1:100 1:1,000 Hypoxia-inducible factor 1-alpha (HIF-1α) Rabbit AB clonal, A11945 1:100 1:1,000 Pyruvate dehydrogenase phosphatase1 (PDP1) Rabbit AB clonal, A6332 1:50 1:500 Phosphoinositide-dependent kinase1 (PDK1) Rabbit Boster, BA4499 1:100 1:1,000 Pyruvate dehydrogenase E1 alpha 1 (PDHA1) Rabbit AB clonal, A1895 1:500 1:5,000 Carnitine palmitoyltransferase 1A (CPT1A) Rabbit Proteintech, 15184-1-AP 1:1,000 β-Actin Mouse Boster, BM0005 1:1,000 Open in a separate window caption a8 Antibodies used for western blotting and immunohistochemistry (IHC) Biochemical analyses Blood was centrifuged at 3,000 rotations per minute for 10 minutes under 4 °C to obtain the serum. .. The serum levels of triglyceride (TG) and total cholesterol (TC) were determined using specific assay kits (Nanjing Jiancheng, Nanjing, China) and applying glycerol-3-phosphate oxidase p-aminophenol (GPO-PAP) and cholesterol oxidase-peroxidase coupling (COD-PAP) methods.

    Immunohistochemistry:

    Article Title: High-fat diet-induced obesity primes fatty acid β-oxidation impairment and consequent ovarian dysfunction during early pregnancy
    Article Snippet: Finally, an enhanced chemiluminescent (ECL) detection kit (Millipore, Germany) was employed to visualize the bands on the membranes. .. The light density and grey level were analyzed and calculated by using ImageJ (Bio-Rad, USA), with β-actin used as a loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Peptide/protein target Species Manufacturer, catalog The concentration of IHC The concentration of Western Blotting Aromatase (CYP19A1) Rabbit AB clonal, ab18995 1:200 1:1,000 Cytochrome P450 family 11 subfamily A member 1 (CYP11A1/P450Scc) Rabbit Boster, BA2120 1:100 1:500 Steroidogenic acute regulatory protein (StAR) Rabbit Bioss, bs-3570R 1:100 1:1,000 Hypoxia-inducible factor 1-alpha (HIF-1α) Rabbit AB clonal, A11945 1:100 1:1,000 Pyruvate dehydrogenase phosphatase1 (PDP1) Rabbit AB clonal, A6332 1:50 1:500 Phosphoinositide-dependent kinase1 (PDK1) Rabbit Boster, BA4499 1:100 1:1,000 Pyruvate dehydrogenase E1 alpha 1 (PDHA1) Rabbit AB clonal, A1895 1:500 1:5,000 Carnitine palmitoyltransferase 1A (CPT1A) Rabbit Proteintech, 15184-1-AP 1:1,000 β-Actin Mouse Boster, BM0005 1:1,000 Open in a separate window caption a8 Antibodies used for western blotting and immunohistochemistry (IHC) Biochemical analyses Blood was centrifuged at 3,000 rotations per minute for 10 minutes under 4 °C to obtain the serum. .. The serum levels of triglyceride (TG) and total cholesterol (TC) were determined using specific assay kits (Nanjing Jiancheng, Nanjing, China) and applying glycerol-3-phosphate oxidase p-aminophenol (GPO-PAP) and cholesterol oxidase-peroxidase coupling (COD-PAP) methods.

    Western Blot:

    Article Title: High-fat diet-induced obesity primes fatty acid β-oxidation impairment and consequent ovarian dysfunction during early pregnancy
    Article Snippet: Finally, an enhanced chemiluminescent (ECL) detection kit (Millipore, Germany) was employed to visualize the bands on the membranes. .. The light density and grey level were analyzed and calculated by using ImageJ (Bio-Rad, USA), with β-actin used as a loading control. table ft1 table-wrap mode="anchored" t5 caption a7 Peptide/protein target Species Manufacturer, catalog The concentration of IHC The concentration of Western Blotting Aromatase (CYP19A1) Rabbit AB clonal, ab18995 1:200 1:1,000 Cytochrome P450 family 11 subfamily A member 1 (CYP11A1/P450Scc) Rabbit Boster, BA2120 1:100 1:500 Steroidogenic acute regulatory protein (StAR) Rabbit Bioss, bs-3570R 1:100 1:1,000 Hypoxia-inducible factor 1-alpha (HIF-1α) Rabbit AB clonal, A11945 1:100 1:1,000 Pyruvate dehydrogenase phosphatase1 (PDP1) Rabbit AB clonal, A6332 1:50 1:500 Phosphoinositide-dependent kinase1 (PDK1) Rabbit Boster, BA4499 1:100 1:1,000 Pyruvate dehydrogenase E1 alpha 1 (PDHA1) Rabbit AB clonal, A1895 1:500 1:5,000 Carnitine palmitoyltransferase 1A (CPT1A) Rabbit Proteintech, 15184-1-AP 1:1,000 β-Actin Mouse Boster, BM0005 1:1,000 Open in a separate window caption a8 Antibodies used for western blotting and immunohistochemistry (IHC) Biochemical analyses Blood was centrifuged at 3,000 rotations per minute for 10 minutes under 4 °C to obtain the serum. .. The serum levels of triglyceride (TG) and total cholesterol (TC) were determined using specific assay kits (Nanjing Jiancheng, Nanjing, China) and applying glycerol-3-phosphate oxidase p-aminophenol (GPO-PAP) and cholesterol oxidase-peroxidase coupling (COD-PAP) methods.

    Electrophoresis:

    Article Title: Short-term methionine deprivation inhibits TCA cycle and regulates macrophage polarization through uncharged tRNA and PDHA1 phosphorylation.
    Article Snippet: Background: Methionine restriction diet has been extensively studied for its beneficial effects on metabolic health and aging.. However, the impact of methionine deprivation on glucose metabolism per se and macrophage functions remains incompletely understood.. Methods: In this study, we analyzed the functional roles of methionine deprivation on glucose flux and macrophage polarization.

    SDS Page:

    Article Title: Short-term methionine deprivation inhibits TCA cycle and regulates macrophage polarization through uncharged tRNA and PDHA1 phosphorylation.
    Article Snippet: Background: Methionine restriction diet has been extensively studied for its beneficial effects on metabolic health and aging.. However, the impact of methionine deprivation on glucose metabolism per se and macrophage functions remains incompletely understood.. Methods: In this study, we analyzed the functional roles of methionine deprivation on glucose flux and macrophage polarization.

    Membrane:

    Article Title: Short-term methionine deprivation inhibits TCA cycle and regulates macrophage polarization through uncharged tRNA and PDHA1 phosphorylation.
    Article Snippet: Background: Methionine restriction diet has been extensively studied for its beneficial effects on metabolic health and aging.. However, the impact of methionine deprivation on glucose metabolism per se and macrophage functions remains incompletely understood.. Methods: In this study, we analyzed the functional roles of methionine deprivation on glucose flux and macrophage polarization.



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    Image Search Results


    Markers of glucose, fatty acid, and ketone metabolism in the remote LV. A Expression of genes associated with glucose metabolism. B Western blot analysis of phosphorylated and total levels of PDH and Akt. C Expression of genes associated with fatty acid and ketone metabolism. D Western blot analysis of phosphorylated and total levels of ACC. * p < 0.05 versus D0 control (Ctrl), # p < 0.05 versus Ctrl from same time point

    Journal: Cardiovascular Diabetology

    Article Title: Distinct immunometabolic signatures of type 1 versus type 2 diabetes in a murine model of myocardial infarction

    doi: 10.1186/s12933-026-03099-y

    Figure Lengend Snippet: Markers of glucose, fatty acid, and ketone metabolism in the remote LV. A Expression of genes associated with glucose metabolism. B Western blot analysis of phosphorylated and total levels of PDH and Akt. C Expression of genes associated with fatty acid and ketone metabolism. D Western blot analysis of phosphorylated and total levels of ACC. * p < 0.05 versus D0 control (Ctrl), # p < 0.05 versus Ctrl from same time point

    Article Snippet: Membranes were then blocked for 1 h (SuperBlockTM Blocking Buffer, Thermo Scientific), and incubated with primary antibodies against PDH (1:1000; CST #3205S), phospho-PDH (Serine 293; 1:1000; CST #31866S), phospho-Akt (serine 473; 1:1000; Cell Signaling Technologies #9271), Akt (1:1000; CST #9272), phospho-ACC (serine 79; 1:1000; CST #D7D11), and ACC (1:1000; CST #3676).

    Techniques: Expressing, Western Blot, Control

    Markers of glucose, fatty acid, and ketone metabolism in the remote LV. A Expression of genes associated with glucose metabolism. B Western blot analysis of phosphorylated and total levels of PDH and Akt. C Expression of genes associated with fatty acid and ketone metabolism. D Western blot analysis of phosphorylated and total levels of ACC. * p < 0.05 versus D0 control (Ctrl), # p < 0.05 versus Ctrl from same time point

    Journal: Cardiovascular Diabetology

    Article Title: Distinct immunometabolic signatures of type 1 versus type 2 diabetes in a murine model of myocardial infarction

    doi: 10.1186/s12933-026-03099-y

    Figure Lengend Snippet: Markers of glucose, fatty acid, and ketone metabolism in the remote LV. A Expression of genes associated with glucose metabolism. B Western blot analysis of phosphorylated and total levels of PDH and Akt. C Expression of genes associated with fatty acid and ketone metabolism. D Western blot analysis of phosphorylated and total levels of ACC. * p < 0.05 versus D0 control (Ctrl), # p < 0.05 versus Ctrl from same time point

    Article Snippet: Membranes were then blocked for 1 h (SuperBlockTM Blocking Buffer, Thermo Scientific), and incubated with primary antibodies against PDH (1:1000; CST #3205S), phospho-PDH (Serine 293; 1:1000; CST #31866S), phospho-Akt (serine 473; 1:1000; Cell Signaling Technologies #9271), Akt (1:1000; CST #9272), phospho-ACC (serine 79; 1:1000; CST #D7D11), and ACC (1:1000; CST #3676).

    Techniques: Expressing, Western Blot, Control